Understanding the Salmonella Inactivation Mechanisms of 365, 395 and 455 nm Light Pulses Emitted from Light-Emitting Diodes

Salmonella is a foodborne pathogen responsible for several outbreaks in low-water activity (aw) foods. Treatment using light pulses emitted from light-emitting diodes (LED) an emerging decontamination method to inactivate pathogens. The objective of this study was understand the antibacterial mechanisms with 365, 395 and 455 nm wavelengths against Typhimurium low-aw conditions. 365 showed better inactivation efficacy S. than pulses. For instance, LED treatment ~217 J/cm2 dose produced reduction 2.94 log (CFU/g) cell counts, as compared 1.08 by same dose. We observed significant generation intracellular reactive oxygen species (ROS) cells after treatments at also membrane lipid oxidation Overall, major role ROS typhimurium